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    Biotechnology Information biotechnology information ncbi sequence
    Biotechnology Information Ncbi Sequence, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ncbi/sequence/pm41662857-188-17-17
    Average 86 stars, based on 1 article reviews
    biotechnology information ncbi sequence - by Bioz Stars, 2026-10
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    Related Articles

    Sequencing:

    Article Title: A comprehensive RNA virome from molluscan transcriptomes reveals extensive diversity and modular genome evolution.
    Article Snippet: .. All data and metadata for these sequence records are publicly 372 available and can be accessed via the NCBI (US National Center for Biotechnology Information) at 373 www.ncbi.nlm.nih.gov. ..

    Next-Generation Sequencing:

    Article Title: A large-scale retrospective analysis reveals the fungal pathogen spectrum across diverse clinical specimens using metagenomic next-generation sequencing
    Article Snippet: .. IFD, invasive fungal diseases; mNGS, metagenomic next-generation sequencing; CSF, cerebrospinal fluid; BALF, bronchoalveolar lavage fluid; NCBI, National Center for Biotechnology Information. ..

    Clinical Proteomics:

    Article Title: A large-scale retrospective analysis reveals the fungal pathogen spectrum across diverse clinical specimens using metagenomic next-generation sequencing
    Article Snippet: .. IFD, invasive fungal diseases; mNGS, metagenomic next-generation sequencing; CSF, cerebrospinal fluid; BALF, bronchoalveolar lavage fluid; NCBI, National Center for Biotechnology Information. ..



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    Phenotypic characterisation of expanded natural killer (NK) cells. NK cells were characterised on Day 14 of expansion. Uniform Manifold Approximation and Projection (UMAP) plots show the 10 distinct meta clusters of NK cells generated via FLOWSOM (a) , with a contour UMAP indicating proportions of NK cells belonging to each cluster (b) . A clustered heatmap visualisation displays the MFI of each indicated marker belonging to NK cells within the corresponding clusters (c) . To model <t>ADCC,</t> <t>PD‐L1</t> was over‐expressed on MCF‐7 cells (d) prior to cytotoxicity assay in the presence/absence of 10 μg/mL avelumab (e) . NK Cell phenotyping, n = 3. Cytotoxicity displayed as individual donors ( n = 4). ** P ≤ 0.005, by multiple paired t ‐tests.
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    Phenotypic characterisation of expanded natural killer (NK) cells. NK cells were characterised on Day 14 of expansion. Uniform Manifold Approximation and Projection (UMAP) plots show the 10 distinct meta clusters of NK cells generated via FLOWSOM (a) , with a contour UMAP indicating proportions of NK cells belonging to each cluster (b) . A clustered heatmap visualisation displays the MFI of each indicated marker belonging to NK cells within the corresponding clusters (c) . To model <t>ADCC,</t> <t>PD‐L1</t> was over‐expressed on MCF‐7 cells (d) prior to cytotoxicity assay in the presence/absence of 10 μg/mL avelumab (e) . NK Cell phenotyping, n = 3. Cytotoxicity displayed as individual donors ( n = 4). ** P ≤ 0.005, by multiple paired t ‐tests.
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    Phenotypic characterisation of expanded natural killer (NK) cells. NK cells were characterised on Day 14 of expansion. Uniform Manifold Approximation and Projection (UMAP) plots show the 10 distinct meta clusters of NK cells generated via FLOWSOM (a) , with a contour UMAP indicating proportions of NK cells belonging to each cluster (b) . A clustered heatmap visualisation displays the MFI of each indicated marker belonging to NK cells within the corresponding clusters (c) . To model <t>ADCC,</t> <t>PD‐L1</t> was over‐expressed on MCF‐7 cells (d) prior to cytotoxicity assay in the presence/absence of 10 μg/mL avelumab (e) . NK Cell phenotyping, n = 3. Cytotoxicity displayed as individual donors ( n = 4). ** P ≤ 0.005, by multiple paired t ‐tests.
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    Phenotypic characterisation of expanded natural killer (NK) cells. NK cells were characterised on Day 14 of expansion. Uniform Manifold Approximation and Projection (UMAP) plots show the 10 distinct meta clusters of NK cells generated via FLOWSOM (a) , with a contour UMAP indicating proportions of NK cells belonging to each cluster (b) . A clustered heatmap visualisation displays the MFI of each indicated marker belonging to NK cells within the corresponding clusters (c) . To model <t>ADCC,</t> <t>PD‐L1</t> was over‐expressed on MCF‐7 cells (d) prior to cytotoxicity assay in the presence/absence of 10 μg/mL avelumab (e) . NK Cell phenotyping, n = 3. Cytotoxicity displayed as individual donors ( n = 4). ** P ≤ 0.005, by multiple paired t ‐tests.
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    Phenotypic characterisation of expanded natural killer (NK) cells. NK cells were characterised on Day 14 of expansion. Uniform Manifold Approximation and Projection (UMAP) plots show the 10 distinct meta clusters of NK cells generated via FLOWSOM (a) , with a contour UMAP indicating proportions of NK cells belonging to each cluster (b) . A clustered heatmap visualisation displays the MFI of each indicated marker belonging to NK cells within the corresponding clusters (c) . To model <t>ADCC,</t> <t>PD‐L1</t> was over‐expressed on MCF‐7 cells (d) prior to cytotoxicity assay in the presence/absence of 10 μg/mL avelumab (e) . NK Cell phenotyping, n = 3. Cytotoxicity displayed as individual donors ( n = 4). ** P ≤ 0.005, by multiple paired t ‐tests.
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    Phenotypic characterisation of expanded natural killer (NK) cells. NK cells were characterised on Day 14 of expansion. Uniform Manifold Approximation and Projection (UMAP) plots show the 10 distinct meta clusters of NK cells generated via FLOWSOM (a) , with a contour UMAP indicating proportions of NK cells belonging to each cluster (b) . A clustered heatmap visualisation displays the MFI of each indicated marker belonging to NK cells within the corresponding clusters (c) . To model ADCC, PD‐L1 was over‐expressed on MCF‐7 cells (d) prior to cytotoxicity assay in the presence/absence of 10 μg/mL avelumab (e) . NK Cell phenotyping, n = 3. Cytotoxicity displayed as individual donors ( n = 4). ** P ≤ 0.005, by multiple paired t ‐tests.

    Journal: Clinical & Translational Immunology

    Article Title: Parallel processing of T cells and natural killer cells to enhance in vivo CAR T cell activity against blood and solid cancers

    doi: 10.1002/cti2.70120

    Figure Lengend Snippet: Phenotypic characterisation of expanded natural killer (NK) cells. NK cells were characterised on Day 14 of expansion. Uniform Manifold Approximation and Projection (UMAP) plots show the 10 distinct meta clusters of NK cells generated via FLOWSOM (a) , with a contour UMAP indicating proportions of NK cells belonging to each cluster (b) . A clustered heatmap visualisation displays the MFI of each indicated marker belonging to NK cells within the corresponding clusters (c) . To model ADCC, PD‐L1 was over‐expressed on MCF‐7 cells (d) prior to cytotoxicity assay in the presence/absence of 10 μg/mL avelumab (e) . NK Cell phenotyping, n = 3. Cytotoxicity displayed as individual donors ( n = 4). ** P ≤ 0.005, by multiple paired t ‐tests.

    Article Snippet: The human PD‐L1 (NCBI 29126) gene with endogenous signal sequence was synthesised by Twist Biosciences and cloned into SfiI sites of pSBbiP—a modified pSBbiGP encoding puromycin resistance but with the original GFP gene removed.

    Techniques: Generated, Marker, Cytotoxicity Assay

    Recycled natural killer (NK) cells enhance CAR T cell therapy of MCF‐7. NSG mice were engrafted with Her2 h i PDL1 hi MCF‐7 cells on Day 0 prior to sequential administration of NK cells (d10) and CAR T cells (d12) (a) . Quality control of both cell types ensured high NK cell purity and transduction efficiency of T cells prior to administration (b, c) . Mice were imaged weekly (d, e) , and callipers were used every other day to monitor tumor burden (f) . Mice were euthanised when radiance and/or tumor volume reached endpoint thresholds (g) . Data from one mouse experiment. n = 4–5 mice per group. Tumor burdens displayed as mean ± SEM. * P < 0.05 by two‐way ANOVA with Bonferroni correction. Survival analysed with Kaplan–Meier, log‐rank mantel‐cox test. ** P ≤ 0.005. White out‐lined box conceals mouse removed from study because of failed tumor engraftment.

    Journal: Clinical & Translational Immunology

    Article Title: Parallel processing of T cells and natural killer cells to enhance in vivo CAR T cell activity against blood and solid cancers

    doi: 10.1002/cti2.70120

    Figure Lengend Snippet: Recycled natural killer (NK) cells enhance CAR T cell therapy of MCF‐7. NSG mice were engrafted with Her2 h i PDL1 hi MCF‐7 cells on Day 0 prior to sequential administration of NK cells (d10) and CAR T cells (d12) (a) . Quality control of both cell types ensured high NK cell purity and transduction efficiency of T cells prior to administration (b, c) . Mice were imaged weekly (d, e) , and callipers were used every other day to monitor tumor burden (f) . Mice were euthanised when radiance and/or tumor volume reached endpoint thresholds (g) . Data from one mouse experiment. n = 4–5 mice per group. Tumor burdens displayed as mean ± SEM. * P < 0.05 by two‐way ANOVA with Bonferroni correction. Survival analysed with Kaplan–Meier, log‐rank mantel‐cox test. ** P ≤ 0.005. White out‐lined box conceals mouse removed from study because of failed tumor engraftment.

    Article Snippet: The human PD‐L1 (NCBI 29126) gene with endogenous signal sequence was synthesised by Twist Biosciences and cloned into SfiI sites of pSBbiP—a modified pSBbiGP encoding puromycin resistance but with the original GFP gene removed.

    Techniques: Control, Transduction